The regulation and maintenance of a tissue’s shape and structure is a major outstanding question in developmental biology and plant biology. In this talk, through iterations between experiments and multiscale model simulations that include a mechanistic description of interkinetic nuclear migration, we will show that the local curvature, height, and nuclear positioning of cells in the Drosophila wing imaginal disc are defined by the concurrent patterning of actomyosin contractility, cell-ECM adhesion, ECM stiffness, and interfacial membrane tension. The biologically calibrated model describing both tissue growth and morphogenesis incorporates the spatial patterning of fundamental subcellular properties. Additionally, the model implements for the first time the dynamics of interkinetic nuclear migration within the simulated pseudostratified epithelium. This includes the basal to apical motion of the nucleus, mitotic rounding, and cell division dynamics. Key characteristics of global tissue architecture, such as the local curvature of the basal wing disc epithelium, cell height, and nuclear positioning, serve as metrics for model calibration. The experiments have shown how these physical features are jointly regulated through spatiotemporal dynamics in the localization of pMyoII, β-Integrin, and ECM stiffness. As the disc grows, there are progressive changes in the patterning of key subcellular features such as actomyosin contractility. The predictions made by the model simulations agree with the observed changes in contractility and cell-ECM adhesion during wing disc morphogenesis. Multiscale modeling approach combined with experiments was also applied to studying stem cell maintenance in multilayered shoot apical meristems (SAMs) of plants which requires strict regulation of cell growth and division. In this talk, the combined approach will be demonstrated through testing three hypothesized mechanisms for the regulation of cell division plane orientation and the direction of anisotropic cell expansion in the corpus.